TCR signal strength and proliferation affect Runx2 expression and histone modifications in CD8+ T cells
نویسندگان
چکیده
Abstract Runx family transcription factors are crucial for the establishment of genetic programs during important cell differentiation stages. Although roles Runx1 and Runx3 in T cells have been extensively studied, role Runx2 has not fully explored. specific deletion leads to a intrinsic defect longevity CD8+ memory population decreased expression markers like TCF1 Eomes. As histone modifiers known binding partners, we propose that may bind associated gene promotors, allowing maintenance chromatin as undergo homeostatic proliferation. To further explore function cells, utilize fl/fl CD4-Cre model deletion. We use an vitroCD8+ stimulation assay which co-culture DC2.4 (a mouse dendritic line) loaded with ovalbumin peptide variants (N4, T4, V4) OT-1 72 hours observe effects changes. Additionally, analyses this system examine modifications activity. In accordance previous data, is inversely correlated TCR signal strength, stimulated low dose/affinity peptides upregulate higher levels Runx2. proliferation dye labeling reveals increases divide continues increase activated rested post-stimulation. Preliminary data also suggest poised vary expression, indicating play cells.
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ژورنال
عنوان ژورنال: Journal of Immunology
سال: 2023
ISSN: ['1550-6606', '0022-1767']
DOI: https://doi.org/10.4049/jimmunol.210.supp.239.22